CLASSIFICATION OF PROTOZOA
Phylum Protozoa is classified into 4 distinct sub-phyla on the basis of type of locomotory organelles found in them. The 4 sub-phyla of phylum Protozoa are as follows:
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Sarcomastigophora
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Sporozoa
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Cnidospora
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Ciliophora
1. SARCOMASTIGOPHORA
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Locomotory organelles may be Pseudopodia or Flagella.
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Nucleus is monomorphic.
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Asexual reproduction is by binary or multiple fission.
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Sexual reproduction occurs by fusion of gametes.
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Sub-phylum Sarcomastigophora is further divided into 3 Super classes i.e.,
-Mastigophora
-Opalinata
-Sarcodina
(a) MASTIGOPHORA (SUPER CLASS)
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Flagella are present as locomotory organelles, so they are also called Flagellata. Flagella also acts as food capturing organ.
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Body is covered by a layer of very thin cuticle or pellicle.
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Nutrition is holozoic, holophytic or saprozoic.
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Asexual reproduction by longitudinal fission.
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Super class Mastigophora has further 2 classes i.e., Phytomastigophora and Zoomastigophora.
(i) PHYTOMASTIGOPHORA (CLASS)
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They possesses chromatophores.
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Only 1 or 2 flagella are found.
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Nutrition is holophytic.
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Ex- Euglena
(ii) ZOOMASTIGOPHORA (CLASS)
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Chlorophyll bearing chromatophores are absent.
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In addition to pseudopodia, more than 2 flagella are present as locomotory organelles.
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Undulating membrane may be present.
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Nutrition is holozoic.
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Ex- Trypanosoma
(b) OPALINATA (SUPER CLASS)
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All are parasites of frogs and toads.
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Body is covered by flagella and arranged in oblique rows over the entire body surface.
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Nuclei are monomorphic.
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Reproduction by simple binary fission or by gamete formation.
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Ex- Opalina
(c) SARCODINA (SUPER CLASS)
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Locomotory organelle is Pseudopodia.
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Body is not usually covered by pellicle.
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Gamete formation is more common.
Super class Sarcodina is further divided into 3 classes i.e., Rhizopoda, Actinopoda, and Piroplasmea.
(i) RHIZOPODA (CLASS)
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Locomotory organelles are lobopodia or filopodia. Axopodia are absent.
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All are creeping forms.
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Ex- Amoeba, Entamoeba
(ii) ACTINOPODA (CLASS)
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Axopodia is found as locomotory organelle.
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Test may or may not be present.
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Reproduction is by both sexual and asexual method.
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Gametes are usually flagellated.
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Ex- Actinophrys
(iii) PIROPLASMEA (CLASS)
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They are small, round, rod shaped or amoeboid forms.
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Parasites of R.B.Cs of vertebrates.
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Ex- Babesa

2. SPOROZOA
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All of them are parasites.
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Locomotory organelles are absent. But gametes may have cilia or flagella.
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Body is covered by thick cuticle or pellicle.
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Asexual reproduction by multiple fission.
Sub-phylum Sporozoa is further classified into 3 classes, such as: Telosporea, Haplosporea, Toxoplasmea.
(a) TELOSPOREA (CLASS)
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Pseudopodia are generally absent. Locomotion is by gliding body movement.
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Reproduction is both sexual and asexual.
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Ex- Plasmodium
(b) TOXOPLASMEA (CLASS)
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Locomotory organelles are absent in all the stages.
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Reproduction is by binary fission.
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Ex- Toxoplasma
(c) HAPLOSPOREA (CLASS)
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Pseudopodia may be present, but flagella is absent.
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Spore cases are present.
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Reproduction is only asexual.
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Ex- Icthyosporidium

3. CNIDOSPORA
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All are parasites.
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Zygotes gives to one or more trophozoites.
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Trophozoites are multinucleated.
Cnidospora has 2 distinct classes, such as:
Myxosporidea and Microsporidea.
(a) MYXOSPORIDEA (CLASS)
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Parasites of fishes.
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Spores are of multicellular origin.
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Spores possesses a bivalved membrane.
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Ex- Myxobolus.
(b) MICROSPORIDEA (CLASS)
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Endoparasites of Arthropods and Vertebrates.
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Spores are of unicellular origin.
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Small spores with univalved membrane.
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Ex- Nosema
4. CILIOPHORA
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Cilia are the locomotory organelles.
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Posses 2 nuclei i.e., one meganucleus (vegetative nucleus) and another micronucleus (reproductive nucleus).
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Nutrition is heterotrophic.
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Asexual reproduction is by binary fission or budding.
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Sexual reproduction is by conjugation, autogamy or by cytogamy.
Sub-phylum Ciliophora has only one class i.e., Ciliata.
(a) CILIATA (CLASS)
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Cilia are present throughout the life.
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Nuclear dimorphism is marked.
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Anal aperature (cytopyge) is permanent.
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Ex- Paramecium